BOD快速测定传感器的微生物膜固定方法研究

Study on the Microbial Film Immobilization Methods for comparing the Biosensors for Rapid BOD Determination

分别采用海藻酸钠包埋法、醋酸纤维素夹层法、戊二醛－牛血清白蛋白交联法等几种物理、化学方法制备固定化微生物膜，并采用自制的微生物ＢＯＤ传感器试验了各种膜对电极响应灵敏度、线性范围、响应时间、稳定性和寿命等性能的影响。比较了不同ｐＨ、温度和交联剂、包埋剂用量下该传感器的性能，获得了较满意的结果。实验表明，以交联法制备的传感器性能最佳，电极寿命达４０ｄ，测定ＢＯＤ值时间为１０－１５ｍｉｎ，方法的重现性和精密度均达ＢＯＤ测定要求。本研究对于实现快速测定ＢＯＤ传感器的商品化具有重要意义。

Several physical and chemical methods, such as the sokium alginate-calcium chloride embedding process, the cellulose acetate sandwiching process and the bovine serum albumen-glutaraldehyde crosslinking process, were used to prepare the immobilized microbial films which were then combined with an oxygen electrode to make the newly developed biosensors for rapid BOD determination. The tests were carried out for the effects of different films on the performances of electrode, including its response sensitivity, linearity ranges, resPOnse time, stability and life-time. The performances of the biosensors which were formed under different process conditions such as PH values, temperatures, cronlinking agentS and the amounts of embedding agentS used were also compared. The results show that the biosensors prepared with the cross-linking process have the most preferred Performances, including an electrode life-time of up to 40 days, a BOD determination time of 10 to 15 minutes, and the reproducibility and accuracy of determinations both in compliance with the requirements of standard BOD determination.