低剂量甲基汞对小鼠胸腺和脾细胞周期进程的影响

Influence of Low Dose Methylmercury on Cell Cycle Progression of Thymocytes and Splenocytes in Mice

雄性昆明小鼠连续饮用含甲基汞浓度为１／１０００ＬＤ_（５０）、１／１００ＬＤ_（５０）、１／５０ＬＤ_（５０）和１／１０ＬＤ_（５０）的自来水９０天，采用ＦＡＣＳｃａｎ流式细胞仪（ＦＣＭ）分析胸腺和脾细胞周期进程。发现除１／１０００ＬＤ_（５０）剂量组外，其余各剂量组从Ｇ０／Ｇ１时相进入Ｓ时相的脾细胞百分数均明显高于对照组（Ｐ＜０．０５），与染毒剂量呈明显正相关；而胸腺细胞周期进程与对照组无明显差异。提示连续经口摄入低剂量甲基汞小鼠脾细胞周期进程加快，细胞ＤＮＡ复制增强。

Male Kunming mice drank continuously tap water contained methylmercury concentration in 1/ 1000LD_(50),1/ 100LD_(50),1/50LD_(50)and 1/10LD_(50) for 90 days.The cell cycle progression of thymocytes and splenocytes was analyzed by a FACScan flow cytometry with software'CELLFIT'.It was found that the percentage of splenocytes with S phase from G0/G1 phase in those groups except 1/1000LD_(50)group was significantly higher than in control(P<0.05),correlated positively with contaminative dose.At the same condition,there was no obvious difference in the cell cycle progression of thymocytes from control.The results suggest that the DN A replication of splenocytes in mice was enhanced significantly by low dose methylmercury intaked continuously via the mouth.