温度休克诱导长牡蛎三倍体的研究

A Research on inducing Ostrea gigas Thunbery Triploids by Thermal Shock

本文采用低温２～９℃和高温３５～３９℃温度休克法，在受精后１０～２５ｍｉｎ进行１０～２５ｍｉｎ处理，诱导牡蛎三倍体。结果表明：各处理组都有不同程度的三倍体产生，其中受精后２０ｍｉｎ开始在４～５℃下处理１５ｍｉｎ诱导三倍体效果最好，诱导率为６８×１０－２，幼贝三倍体率为６０．５×１０－２，生长速度处理组壳高明显超过对照组，而壳长生长和幼虫死亡率无明显差异。

This study was designed to induce triploid oysters by cold shocks (2-9℃) and heat shocks (35-39℃) initiates at siffenent fimes for different durationns after fertilizotion. Triploids can be found in each treated group. Cold shock at 4-5℃ for 15 minutes mitiated 20 minutes after fertilization yielded the best results of rates of induction (over68% triploids, in young oysters 60. 5% triploisd). Shell heihgt increased faster in the treated groups than in the control groups but no obvious difference were shown in the growth of shell length and mortalities of the larvae. The larvae had an obvious growth increscent in the treated groups compared with the control groups.