Iloprost对内皮细胞抗血栓功能的影响

Effects of I1oprost on Antithrombus Function of Endothelial Cells

以体外培养的猪主动脉内皮细胞（ＥＣ）为模型研究Ｉ１ｏｐｒｏｓｔ对ＥＣ抗血栓功能的影响。用放免法、发色底物法测定培养液中６－酮－ＰＧＦ１ａ（６－ｋｅｔｏ－ＰＧＦ１ａ），血栓素Ｂ２（ＴＸＢ２）含量，纤溶酶原激活物（ＰＡ），纤溶酶原激活物抑制物（ＰＡＩ）活性及ＥＣ环磷酸腺苷（ｃＡＭＰ）含量。结果表明：Ｉ１ｏｐｒｏｓｔ可以使ＥＣ生成６－ｋｅｔｏ－ＰＧＦ１ａ，ｃＡＭＰ含量显著增多，ＴＸＢ２含量显著减少，并使ＰＡ活性升高，ＰＡＩ活性降低。提示：Ｉ１ｏｐｒｏｓｔ通过促进抗凝、纤溶作用，能够增加ＥＣ的抗血栓功能。

y using cultured porcine aortic endothelial cells(EC)as an experimental mo-del in vitro 6-keto-pGF1α,TXB2 content, plasminogen activator (PA), plasmino-gen activator inhibitor(PAI)activity in the supernatant and cyclic AMP level in ECwere measured with RIA and the method of chromogenic substrates.The results showed: after administration of I1oprost 6-keto-pGF1α,cyclic AMPcontent and PA activity ,were significantly higher,TXB2 content, PAI activitywere remarkably lower than control group. These effects were dose dependent. These findings indicated that I1oprost might act as a prospective drug for anti-thrombosis through promoting anticoagulation and fibrinolysis function, increasingantithrombus action of EC.