摘要
应用细胞封接式膜片钳方法,在Wistar大鼠的单个心室肌细胞上,观察了人参二醇组皂甙Rc(ginsenoside-Rc),Rd(ginsenoside-Rd)200μg/ml对钙通道单通道活动的影响,并与钙通道阻滞剂异搏定37.5μg/ml、钙通道激动剂BAYK8644μmmol/L对照,证明Rc对钙通道有阻滞作用;而Rd则无此作用。用电子自旋共振法(electronspinresonance,ESR)测定了培养心肌细胞的自由基含量,Rc30μg/ml能显著抑制黄嘌呤0.42mmol/L-黄嘌呤氧化酶5.3nmol/L所致的自由基含量的增多:而Rd30μg/ml对其影响则不明显。
he effects of Rc, Rd 200μg/ml to single Ca2+channel on ventricular myocar-diocytes of Wistar rats Were recorded using the cell-attached configuration of thepatch clamp technique,and compared with calcium channel blocker verapamil 37.5μg/ml and calcium channel activator BAY K 8644 5μmmol/ml. Panaxadiol saponinmonomer Rc 200μg/ml inhibited the activities of T,L and B type of calciumchannel significantly,whill Rd 200μg/ml have no this effect. Electron spin reso-nance was used to measure the free radical contents of the cultured cardiomyocytes.Rc 30 μg/ml signif icantly reduce the increased free radical content indued by xanth-ine 0.42mmol/L-xanthine oxidase 5. 3nmol/L, but Rd 30μbg/ml no this effect.
出处
《基础医学与临床》
CSCD
1994年第4期46-50,共5页
Basic and Clinical Medicine
关键词
钙通道
人参
皂甙
电子自旋共振
calcium channel ginsenoside patch clamp BAK Y 8644 electron spin resonanceCentral Laboratory of PhysiologyDepartment of Pharmacology