用SDS—PAGE、Western blot和ELISA方法检测猪囊虫囊液中有效抗原的研究

Effective Antigens in Cystic Fluid of Cysticercus Cellulosae as Determined by SDS-PAGE, Western Blot and ELISA Techniques

用SDS-PAGE、Western blot和ELISA方法检测猪囊虫囊液中的有效抗原,结果猪囊虫囊液中有8条抗原区带被猪囊虫病患者血清中抗体识别,分子量分别为255、185、105、69、64、60、48、和41kDa。其中第至3第7条免疫活性较强,含量较高,为有希望用于研制猪囊虫病疫苗的抗原分子。

The result of detection of effective antigens contained in cystic fluid of cysticercus cellulosae with SDS-PAGE, western blot and ELISA tech- niques, Eight antigen bands were recognized with antibodies in sera of cysticercosis patients, The molecular weights of the 8 bands were 255, 185, 105, 69, 64, 60, 48, and 41 kDa respectively. The 3rd to 7th bands appeared to be broader and more beeply stained than other bands, indica- ting that they may have stronger immune activities. If so, they may be better candidates for prepareing cysticereosis vaccines.