大鼠肾上腺髓质组织的冰冻储存和移植

Freezing and Transplantation of Adrenal Medulla Tissue in Rat

通过观察移植物的细胞形态和检测移植后巴金森氏模型鼠旋转行为的变化,探讨了低温储存肾上腺髓质组织的可行性。肾上腺髓质组织取自成年Sprague--Dawley大鼠,以每分钟降温1～2℃的速率冰冻,在液氮中储存1周,然后移植于6羟多巴胺损毁一侧黑质的模型鼠尾状核内。移植后Apomorphine诱发模型鼠的旋转行为有不同程度改善;冰冻储存的肾上腺髓质组织在细胞形态和染色特性上未发生变化;荧光观察表明移植物仍然保持产生儿茶酚胺的机能。这提示低温冰冻没有改变肾上腺髓质组织的生物学特性,可应用冰冻储存肾上腺髓质组织的方法建立组织库。

In this study, the feasibility of storing adrenal medulla tissue at low temperature was investigated by observing the morphology of graft cells and testing the change of rotation behavior in model rat of Parkinsonism after transplantation. The medulla tissue taken from adult Sprague-Dawley rats, frozen at the rate of decreasing 1 to 2℃ per minute, stored at -196℃ for one week, were transplanted into the caudate nucleus of host brain, where the unilateral substantia nigra was damaged by 6-hydroxydopamine. Test for rotation behaviour induced by apomorphine showed that the rotation reduced partly after operation; Histological examination revealed that the shapes of graft cells and the feature of staining did not change after frozen; histo- fluorescent observation showed the graft cells still maintain the function of producing catecholamine. It is suggested that freezing to low temperature does not adversely affect the biological character of adrenal medulla tissue, and the frozen-stored method may be utilized for setting up adrenal medulla tissue bank.