摘要
以E coli C300为受体菌,利用人乳头瘤病毒11型DNA与pBR322的重组质粒(pBR322-HPV11DNA),成功地进行了HPV11DNA的转化,经筛选获得61株重组子,随机对15株转化子以碱变性法及清亮裂解法抽提了pBR322-HPV11DNA的重组质粒,纯化后经BamHI酶切及琼脂糖凝胶电泳,以λDNA/HindⅢ作参考分子量测定,初步结果证实存在有分子量为5.5×10~6(约8.1kb)的HPV11DNA。这为HPV11DNA的分子扩增,基因探针的制备及HPV11型量鳞癌的研究提供了方便。
Successful transformation have been mede by using the recombinant plasmid of human papillomavirus type11 DNA(HPV11DNA) and pBR322 plasmid vector with E coli C600 as the receptor bacteria. Sixty-one strains were obtained and fifteen strains were selected randomly for further identification. The pBR322- HPV11DNA plasmid were isolated by both the alkaline denature method and the clear cleavage method and cut by BamHI. Then the agarose gel electrophoresis has been done. The molecular weight of HPV11DNA was assayed by compairing with the reference DNA of standard bacteriophage which was cleaved with HindⅢ. The results showed there was the DNA band of HPV11 DNA with the MW of approximately 5.5×10~6dts and 8.1kb. So it is convenient for the further amplification of the pure HPV11DNA and production of the genetic probe for exploring the association of the HPV11 infection with the development of sequamous cancers.
基金
国家自然科学基金
