在裸鼠体内连续传代的人类大肠癌HT-29细胞系分化程度逐渐增高现象的发现及研究

Changes of Differentiation of Human Colonic Adenocarcinoma HT-29 Cell Line Serially Passaged in Nude Mice

对在裸鼠体内连续传代的HT-23细胞系对宿主间质细胞影响的研究中,偶尔发现随着传代次数的增加,移植瘤细胞的分化程度逐渐增高,移植瘤逐渐由未分化癌变为高分化腺癌,表现为瘤细胞呈腺腔样排列,腺腔内有粘液分泌,间质细胞增多。第5代移植瘤出现了类似结肠绒毛样结构。带瘤裸鼠血清中单位瘤体积癌胚抗原(CEA)含量也逐渐下降。这一结果提示:异体恶性细胞在免疫缺陷动物体内连续传代有可能导致恶性的逆转。

In the experiment of induction of transformation of host stroma cells in nude mice by transplanted human colonic adenocarcinoma HT-29 cell liner, we discovered accidentally that the serial passage of the HT-29 cell line in nude mice caused the differentiation of human tumor cells. There appeared a lot of granular cavities, mucus-likem material secreted by differentiated adenocarcinoma cells and rich connective tissue in the sections of 3rd and 4th passage. Moreover, colonic villi-like structures can be seen in sections of 5th passage. We also found that the amount of carcinoembryonic antigen(CEA) per unit volume of tumor in serum of tumor bearing animals decreased as the number of passage was incerased.