家兔角膜硷烧伤微量元素Zn、Cu含量及角膜胶原代谢变化

Alteration of the Trace Element (Zn, Cu ) Contents and Collagen Metabolism in Rabbit Corneas which were Burned artificially by Alkali

选体重2.5kg左右健康家兔48只,随机分为2组。实验组兔(32只)双眼角膜贴附浸有0.5mol/L NaOH液的滤纸50s后致中度碱烧伤,对照组兔(16只)不予处理。观察期间(50d),实验组兔主要呈现间歇性角膜溃疡,其角膜胶原含量和血清游离Hyp含量变化证实,角膜溃疡的发生是由于烧伤后胶原合成减少和分解加强所致;角膜Zn含量与胶原酶活性有内在联系,而Cu含量对胶原酶活性无影响。据胶原分解高峰出现时间,临床应用胶原酶抑制剂的时机宜在烧伤后7d之前。

Forty-eight healthy rabbits were randomly divided into twogroups. In group Ⅰ, 32 rabbits, whose bilateral corneas were burned by sti-cking them with NaOH solution-soaking filter paper strips for 50 s, wereused as experimental model. Group Ⅱ (including 16 rabbits) is control.Theobservation period lasted for 50 day. Intermittent corneal ulceration wasthe major clinical manifestation. Based upon the alteration of the contentsof corneal collagen and serum free Hyp, it was confirmed that cornealulceration resulted from burn is due to the reduction of collagen synthesisand increment of its decomposition. There is an internal relation betweenthe content of Zn and the activity of collagenase, but Cu has no influenceon its activity. According to the appearance time of collagen decomposition climax, the collagenase inhibitor should be used within one weekafter burning.