奶牛冷冻胚胎分割和分割后半胚冷冻技术

The Technique of Frozen-embryo Bisection and Demi-embryo Freezing in Dairy Cattle

用简易分割方法,分割奶牛冷冻胚胎16枚,获得半胚32枚,分割成功率为100％(16/16),可移植半胚29枚,移植于16头受体牛,到第3个情期未返情者经直肠检查有6头妊娠,妊娠率为37.5％(6/16)。其中,在分割前或者分割后经恢复培养0.5～2h再移植的10头受体牛,5头妊娠;而未经恢复培养,分割后直接移植的6头受体牛中,只有1头妊娠。移植后3个月,直肠检查确定2头流产。已有1头受体黄牛生出1头奶牛牛犊(母)和1头受体奶牛产1头奶牛牛犊。其余的2头妊娠受体牛将于9月份产犊。此外,用简易分割法分割奶牛胚胎5枚,得到半胚10枚,裸半胚直接冷冻,解冻后回收可移植半胚5枚,移植于4头受体牛,无一头妊娠。结果表明,冷冻胚胎的分割半胚优于分割后冷冻半胚移植效果;冷冻胚胎分割前或者分割后恢复培养移植优于未经恢复培养而直接移植;简易分割法可应用于冷冻胚胎的分割。

A com parative study on the frozen-embryo bisection and demi-embryo freezing for finding a simple method to raise the embryo utiliza-tion was made. Sixteen frozen embryos had been bisected and 29 demi-embryos were transferred. The pregnancy rate was 37.5% (6/16). Fiveembryos (A, B grade) were bisected and then frozen. When the 5 demi-em-bryos were transferred to 4 recipients no one of them produced prognancy.The results showed that the effect of frozen embryo bisection method wassuperior to that of bisected embryo freezing one, and the restoring cultiva-tion of frozen embryo before or after bisection has a significant influenceon the utilization and pregnancy rates.