酶联免疫吸附试验诊断鹿结核病

Research of Diagnosing Deer TB with ELISA

本文报告了应用酶联免疫吸附试验（ＥＬＩＳＡ）诊断鹿结核病的研究中所进行的抗原筛选、各要素最适稀释度、最适作用条件以及所选用抗原的特异性和敏感性等诸方面较系统地研究过程和方法、结果表明，５种抗原在获取方法、特异性和敏感性的比较中，以ＰＰＤ为最佳，从而建立了以ＰＰＤ为抗原的ＥＬＩＳＡ检测鹿结核病的血清学诊断程序。通过对１２６８头鹿ＥＬＩＳＡ检测结果与变态反应结果对比证明：两种诊断方法的阳性符合率为８２％，二者不能互相代替，联合应用可提高检出率的结果。

The research results showed that sensitivity and speicity of PPD in the 5 antigens are the highest. Therefore, the PPD antigen ELISA was established to dagnose deer TB.The positive coincide between ELISA and hypersensitive reaction was 82% when 1286 deers were detected with two methods.The combination of two methods can achieve high positive rate.