摘要
对Marmarou等提出的溴代苯──煤油密度梯度方法,在取材方式,比重柱的制备与标定,皮层样品的标准化和短时贮存等方面进行了改进,提高了该技术在实际应用中的准确性和可重复性。与经典的干—湿重方法相比较具有良好的相关特性(P<0.01),具有直观、简便和更加敏感的特点。利用上述密度一梯度方法,测定了沙土鼠急性脑缺血不同时程大脑皮层比重的变化,实验表明:在一定条件下随着缺血时间的延长,缺血性皮层水肿进行性加重。结果和利用干—湿重法所得结果相吻合。
We have improved the density and stair method that was used for measuring the ceredral edema,which has been ameliorated its accuracy and reappearance. The results which is form the improved method are remarkably relationship as compared with that of traditional method(r=0.946,P<0.01).The specific gravity of ischemic cerebral cortex was measured by using the improving method.The results suggested that the ischemic cortex edema become worseu,when the ischemic time was prolonged.And this improving density and stair method was a better means,which was applied in iachemic cerebral edema research.
出处
《济宁医学院学报》
1994年第2期15-18,共4页
Journal of Jining Medical University
关键词
溴代苯
煤油
脑水肿
测定
比重
Bromobenzene
Kerosene
Cerebral edema
Measure
Specific gravity