性病患者生殖器单纯疱疹病毒的快速诊断与分型

RAPID DIAGNOSIS AND TYPING OF GENITAL HERPES SIMPIEX VIRUS INFECTION IN PATIENTS WITH VENERRAL DISEASE

本研究采用常规细胞培养、细胞培养加免疫酶染色及ＥＬＩＳＡ方法对８１份临床不同性病患者生殖器标本进行了单纯疱疹病毒（ＨＳＶ）快速诊断及分型。结果表明，不同稀释度ＨＳＶ接种常规细胞培养中，接种后２４ｈ１Ｏ（－１）～１０（－２）稀释度６个培养瓶均阳性，而细胞培养加免疫酶染色接种病毒后８ｈ可在１Ｏ（－３）滴度瓶中检测到ＨＳＶ抗原。目前生殖器ＨＳＶ感染仍以ＨＳＶ－２为主占９０．６％，其次ＨＳＶ－１感染逐渐增多（９．４～１２．９％）。

The study was made by using Conventional Cell Culture(CCC),cell culture plus immunoperxidase staining and ELISA method for rapid diagnosis and typing of 81 clinical genital herpes simplex virus infection samples. The results showed that 10(-1)～12(-2) titers cell tubes were all positive after different virus titer at 24h in CCC method, While 10(-3) titer tube was positive at 8h after injecting by using cell culture plus immunoperoxidase staining. Now, HSV-2 infection was still the majority of genital herpes simplex virus infections,but patients with genital HSV-1 infection were gradually increasing.