人胃癌肿瘤浸润淋巴细胞(TIL)两种分离诱导培养方法抗肿瘤作用的比较

Comparision of Antitumor Effect of Tumor Infiltrating Lymphocytes TIL, Prepared from Human Gastric Carcinoma Separated in Two Different Methods

对人胃癌肿瘤浸润淋巴细胞（TumorInfiltratingLymphocytesTIL），用直接分离和酶消化分离两种方法进行抗肿瘤作用比较，观察到直接法得TIL细胞数低，增殖快，酶消化法得TIL细胞数较多，增殖慢，两种方法制备的TIL细胞抗肿瘤效果无差异。有些肿瘤组织标本，质地坚硬，用直接法得TIL细胞太少，难以满足实验要求，本文认为两种方法合用，可互补其各自不足，是可取的。

This study compared the antitumor effect of tumor infiltrating lymphocytes(TIL)of gastric carcinoma seperated by two different methods of direct separation and digested separation by enzymes. It was demonstrat ed that TIL from direct separation was less in amount,but proliferated fast,and TIL from enzymatic digestion was more in amount,but proliferated slowly. The antiturnor effect of TIL by both two methods was the same. In some kinds of solid tumor,TIL obtained by direct separation was too little in amount to meet the demand of experiments. TIL might be probably the filled up by the other las the authors suggested shortcomings in either method of preparation of.