摘要
报道了草瑚珊愈伤组织生长和次生代谢物与光、温度、ｐＨ不同的物理因子培养条件的关系。结果表明，暗培养的愈伤组织生长和代谢物含量较高，而蓝光在草珊瑚愈伤组织培养中，表现了蓝光效应，诱导出一个优良的愈伤组织系，其相对增长率和总黄酮月产率分别为７５９％，１．８６８ｇ／Ｌ。最佳培养温度为２６℃恒温，最适愈伤组织生长的培养基ｐＨ值为５．５；而最适愈伤组织代谢次级产物的生产培养基ｐＨ值为６．０。
This paper reported the relationship between callus growth and secondary metabolite of Sarcandra glabra with different culture conditions of lights, temperatures and pH values. The results demonstrated that the callus growth of dark culture showed better than white light and red light; but it was the blue light effect in callus culture of Sarcandra glabra.A good callus strain was induced by blue light culture, the relative increasing rate reached seven hundred fifty nine percent and yield of flavonoid glycosides per month 1 868 mg/1, the optimum temperature was around 26℃ and the optimume pH of callus growth was 5.5 but the optimum pH of callus production was 6.0.
出处
《江西科学》
1994年第2期85-89,共5页
Jiangxi Science
基金
江西省自然科学基金
关键词
草珊瑚
愈伤组织
组织培养
光
温度
Sarcandra glabra
Blue light effect
Callus strain
Relative increasing rate
Yield of flavonoid glycosides per month
