离子交换法分离L－谷氨酰胺的工艺研究

STUDY ON THE PROCESS OF ISOLATING L-GLUTAMINE BY ION EXCHANGING

就应用强酸性阳离子交换树脂（Ｂ）及强碱性阴离子交换树脂（Ｇ）分离提纯发酵液中Ｌ－谷氨酰胺（Ｌ－Ｇｌｎ）的工艺条件进行了研究，通过单因子及正交试验确定离子交换分离法分离Ｌ－Ｇｌｎ的较佳条件为：强酸性阳离子交换树脂分离Ｌ－Ｇｌｎ的上柱液ｐＨ为３．５，洗脱剂氨水的浓度为０．０３ｍｏｌ／Ｌ，洗脱速度为树脂体积的０．６５％；强碱性阴离子交换树脂分离Ｌ－Ｇｌｎ的上柱液的ｐＨ为８．０，洗脱剂盐酸的浓度为０．０５ｍｏｌ／Ｌ，洗脱速度为树脂体积的１．１５％；使用上述条件提取Ｌ－Ｇｌｎ的得率为３０％左右。

In this article,we studied the process conditions of isolating and purifying L-Glutamine(L-Gln) from the fermentative liquid by using strong acidic cation exchange resin(B) and alkaline anion exchange resin(G).By single-factor test and rhombic test,better conditions of isolating L-Gln with ion-exchange resin method was found:L-Gln was isolated by using resin(B) with the pH of fermentative broth before putting it into the resin column being adjusted to 3.5,NH4OH elute 0.03 mol/L and eluating speed at 0.65% of the bulk of the resin;then L-Gln was isolated through resin(G) with pH of the fermentative liquid being adjusted to 8.0,NH4OH elute 0.05 mol/L and eluating-speed at 1.150% of the bulk of the resin.Applying the above conditions,L-Gln yielded at a rate about 30%.