聚合酶链反应单链构象多态性检测乳腺癌中p53基因突变

USk OF POLYMERASE CHAIN REACTION SINGLE STRAND STRAND CONFORMATIONPOLYMORPHISM TECHNIQUE TO DETECT p53 GENE MUTATATIONS IN BREASTCANCERS

应用聚合酶链反应单链构象多态性（ｐｏｌｙｍｅｒａｓｅｃｈａｉｎｒｅａｃｔｉｏｎｓｉｎｇｌｅｓｔｒａｎｄｃｏｎｆｏｒｍａｔｉｏｎｐｏｌｙｍｏｒｐｈｉｓｍ，ＰＣＲ－ＳＳＣＰ）方法，对２４例原发性乳腺癌肿瘤组织基因组ＤＮＡ进行了分析，结果表明：其中７例存在ｐ５３基因的突变，突变频率约为３０％。同时对其中１０例进行Ｓｏｕｔｈｅｒｎｂｌｏｔ分析，有２例在ｃｈｒ１７Ｐ上存在等位基因的缺失，而其另一等位基因上均存在基因突变。

We utilized the polymerase chain reaction single strand conformation polymorphism(PCR-SSCP ) technique to detect twentyfour primary breast tumors mutations of p53 including exons 5-9, theresults indicated that seven mutations in the p53 gene were detected in 24 tumors(30%).Loss of het-erozygosity on chromosome 17P was detected by southern blot in two of ten tumor DNAs, the two tumorDNAs all contained mutations in the remaining allese