山莨菪碱对缺血再灌注损伤心肌膜脂质中主要脂肪酸含量影响的研究

FFECT OF ANISODAMINE ON THE CONTENT OF PRINCIPAL,FATTY ACIDS INTHE MEMBRANE LIPIDS OF ISCHEMIA REPERFUSED MYOCARDIAL INJURIES

麻醉大鼠心肌缺血１５ｍｉｎ，再灌１０ｍｉｎ，再灌前１ｍｉｎ分别静注山莨菪碱（Ａｎｉ）３．０ｍｇ·ｋｇ￣－１和５．０ｍｇ·ｋｇ￣－１、生理盐水（ＮＳ）１．０ｍｌ·ｋｇ￣－１、ＳＯＤ７５Ｕ·ｋｇ￣－１，然后取出心脏，用气相色谱法测定心肌膜脂质中主要脂肪酸含量。实验证明在缺血再灌注损伤心肌膜脂质中，主要不饱和脂肪酸含量明显降低（Ｐ＜０．０ｌ），Ａｎｉ能阻止其降低（Ｐ＜０．０１），Ａｎｉ与ＳＯＤ有类似作用（Ｐ＜０．０１）。

In the present experiment, anesthesized rats were subjected to 15min of myocardial is- chemia induced by coronary ligation and followed by 10 min of reperfusion. Moreover, 1 min prior to reperfusion,3.0 and 5.0mg/kg anisodamine,1.0 ml/kg normal saline and 75 U/kg superoxide dismutase were intravenously administered, respectively, Then the animals were sacrificed for isolation of hearts and the content of principal fatty acids in the membrane lipids of reperfused myocardium waxdetermined by gas chromatography. The findings showed that there was a marked decline in the level of princlpal unsatu- rated fatty acids (P<0. 01) and anisodamine could terminate the decrease(P<0. 01). It was also found that superoxide dismutase exerted similar effect to anisodamine.