摘要
麻醉大鼠心肌缺血15min,再灌10min,再灌前1min分别静注山莨菪碱(Ani)3.0mg·kg ̄-1和5.0mg·kg ̄-1、生理盐水(NS)1.0ml·kg ̄-1、SOD75U·kg ̄-1,然后取出心脏,用气相色谱法测定心肌膜脂质中主要脂肪酸含量。实验证明在缺血再灌注损伤心肌膜脂质中,主要不饱和脂肪酸含量明显降低(P<0.0l),Ani能阻止其降低(P<0.01),Ani与SOD有类似作用(P<0.01)。
In the present experiment, anesthesized rats were subjected to 15min of myocardial is- chemia induced by coronary ligation and followed by 10 min of reperfusion. Moreover, 1 min prior to reperfusion,3.0 and 5.0mg/kg anisodamine,1.0 ml/kg normal saline and 75 U/kg superoxide dismutase were intravenously administered, respectively, Then the animals were sacrificed for isolation of hearts and the content of principal fatty acids in the membrane lipids of reperfused myocardium waxdetermined by gas chromatography. The findings showed that there was a marked decline in the level of princlpal unsatu- rated fatty acids (P<0. 01) and anisodamine could terminate the decrease(P<0. 01). It was also found that superoxide dismutase exerted similar effect to anisodamine.
出处
《解放军医学杂志》
CSCD
北大核心
1994年第5期365-367,共3页
Medical Journal of Chinese People's Liberation Army
关键词
山莨菪碱
心肌
再灌注损伤
脂肪酸
Anisodamine
Myocardial reperfusion injury
Cell membrane lipids
Fatty acids
Gas
chromatography