黑龙江立克次体的免疫印迹分析

ANALYSIS OF IMMUNOBLOT OF RICKETTSIA HEILONGJANGI

本文应用蛋白免疫印迹试验对黑龙江立克次体５４与３６株，国内分离株ＪＨ－７４、Ｈｕ－８４株及国际标准株西伯利亚２４６株，康氏Ｓｉｍｋｏ株，小蛛Ｋａｐｌａｎ株等斑点热群立克次体作抗原多肽分析。图谱表明，黑龙江立克次体５４与３６株两者完全一致，具有两条分子量为２１５ＫＤ、６６ＫＤ的主要抗原多肽带，和其它立克次体有明显区别，说明在抗原结构上的特异性。ＪＨ－７４、Ｈｕ－８４与西伯利亚２４６三者相同，同属一个种。斑点热群不同种间的抗原多肽带的数量与分子量互有差异。结果说明黑龙江立克次体可能为斑点热群一新种。

e studied the differences among Rickettsia Heilongjiangi 54 and 36,strain JH-74,Hu-84,R. Siberica 246,R. Conorii Simko and R. Akarii Kap-lan by immunoblot.The profile showed Rickettsia Heilonjiangi 54 and 36 areexactly the same, they all have 2 major bands with molecular weights of215 KD and 66 KD respectively. This is obviously different from other ric-kettsiae indicating difference in the antigen structure. Strain JH-74, Hu-84and R Siberica 246 are similar,they belong to the same species,The numb-er and molecular weights of major antigen polypeptide from different speciesof spotted fever group rickettsiae are different.This further demonstratedthat Rickettsia Heilongjiangi is a new species of SFG rickettsia.