红细胞分化因子对小鼠红白血病细胞系生长的抑制作用

THE INHIBITORY EFFECT OF ERYTHROID DIFFERENTIATION FACTOR(EDF)ON THE GROWTH OF MOUSE ERYTHROLEUKEMIA(MEL)CELLS IN VITRO

分析了红细胞分化因子（ＥＤＦ）对小鼠红白血病（ＭＥＬ）细胞生长的抑制作用。当ＥＤＦ剂量为０．１２ｇ／ｍｌ时，处理２ｄ后的３Ｈ－ＴｄＲ掺入率比对照组降低５０％，第３ｄ的细胞增殖抑制率达８２％；在软琼脂上形成的集落数为对照组的０．５２％。同时，处于细胞周期不同时相的细胞数量发生显著改变．Ｇ０加Ｃ１期细胞数比对照组增加５５．６６％，Ｓ期细胞数增加１４．３％，而Ｇ２和Ｍ期细胞数则减少２８．８％。此外，细胞电泳迁移率也减少到对照组的５０％。以上结果说明，ＥＤＦ对ＮＩＥＬ细胞的增殖有抑制作用。

The inhibitory effect of EDF on the growth of MEL cells in vitro has been analyzed in this study.We found that when the final concentration of EDF was 0.12μg/ml,the cell growth was inhibited,the 3H-TdR incorporation rates decreased by 50% at the 2nd day,the growth inhibitory rate of the cells was 82% at 3rd day,and the cells,colony formation ability on soft agar was 0.52% of the contrast.When the concentration of EDF was 0.10μg/ml,the flow cytometry experiment showed that the G0 and G1 phase cells were increased by 55.6%,the S phase cells were increased by 14.3%,but the G2 and M phase cells were decreased by 28.8%.In addition,the cell electrophoresis mobility rate also decreased to 50% of the original one after treated with EDF.All the results indicated that EDF possess inhibitory effect on the proliferation of the MEL cells.