丁胺卡那霉素对豚鼠内耳毒性影响的组织病理学观察

OBSERVATION ON HISTOPATHOLOGICAL CHANGS OF INNER EAR INDUCED BY AMIKACIN IN GUINEA PIGS

通过全耳蜗铺片及前度迷路取材,运用光镜和电镜观察豚鼠丁胺卡那霉素中毒后内耳的病理变化:结果见耳蜗明显受损,以底回为重.螺旋器外毛细胞坏死较多,而内毛细胞及支持细胞病变较轻.前庭椭圆囊耳石细碎、毛细胞纤毛粘连、脱落;壶腹嵴中央区纤毛也缺失.实验对客观评价丁胺卡那霉素的内耳毒性及有效地预防其中毒性耳聋的发生提供了实验形态学资料.

With the surface preparation of membranous labyrinth, the histopathological changes of inner ear by amikacin in guinea pigs were observed under phase-contrast microscope and scanning electron microscope. The results indicated that cochleas were severely damaged, particularly in basal turns. The outer hair cells in Corti's organ were destroyed to a greater extent while inner hair cells and supporting elements were well preserved. Simutaneously, degenerated otoliths and fusing stereocilia appeared in the utricles and the loss of sensary cilia in the central part of cristae ampullared. This experiment presents an objective evaluation on the ototoxicity of amikacin and some morphological data of the prevention of hearing loss.