人参总皂甙对培养鼠肝枯否细胞免疫活性的影响

A IMMUNOCOMPETENCE STUDY OF GINSENOSIDES IN THE CULTURE OF RAT HEPATIC KUPFFER CELL IN VITRO

分离Wistar乳鼠肝枯否细胞并作体外培养,取细胞生长良好的培养瓶分为正常组,损伤组,实验组及阳性对照组.正常组为接种后3天定时更换培养液者;损伤组为更换5mol/L浓度ccI4培养基后继续培养60min者;实验组为损伤同时加入0.5%人参总皂甙2滴者;阳性对照组为损伤同时加入PHA100μg/ml者.将各级培养瓶继续培养60min后取出细胞生长盖玻片,检测各组枯否细胞免疫活性.结果,正常组与损伤组,实验组与损伤组之间有显著差异(P<0.01),而正常组与实验组无明显差异,按近阳性对照组.电镜观察,正常组枯否细胞表面伸出许多突起,胞质内含吞噬的绵羊红细胞,溶酶体、线粒体很丰富,粗面内质网发达,可见膜被囊泡和髓样结构及吞噬体和溶噬体,损伤组枯否细胞吞噬的绵羊红细胞数目比正常组少或无吞噬,细胞器的病理改变不很明显;实验组枯否细胞吞噬的绵羊红细胞数目比损伤组明显增加,接近正常组及阳性对照组.

Hepatic Kupffer cells of Wister rat were isolated and cultured in vitro. They were divided into four groups, the normal group, the injuried group, the experimental group and the control group. The immunocompetence of the cells revealed that there was significant difference between the normal and the injuried group, and between the experimental and the injuried group, P<0.01, but no significant difference between the normal and the experimental group. In the normal group, many processes were seen under the elelctron microscope on the Kupffer cells'surface with phagocytized sheep red blood cell (SRBC), abundant lysosomes, mitochondria, RER, membrane-coating vesicles, medullary structures and phagosomes, which was not obivious in the injuried group. The number of phagocytized SRBC increased evidently in the experimental group, which was approximate to that in the normal and the positive control group.