HBV－DNA套式一次PCR方法的建立

Establishment of HBV-DNA nested polymerase chain reaction at one time

应用乙型肝炎病毒ＤＮＡＣ基因区的一对外引物（ＨＢＣ＿１和ＨＢＣ＿２，扩增片段长度为５６６ｂｐ）及一对内引物（ＨＢＣ＿３和ＨＢＣ＿４，扩增片段长度为３０１ｂｐ）对不同稀释度的ＨＢＶ－ＤＮＡ（ａｄｒ亚型）进行套式一次ＰＣＲ扩增，结果表明灵敏度可达到１０ａｇ水平。此方法快速、简便、灵敏、材异，与分子杂交的灵敏度相当。

With two outer primers and two inner primers covering C gene region of HBV DNA, the nestedpolymerase chain reaction was used to amplify 566 bp and 301bp DNA fragments at one time. Its sensitivitv isabout 10ag at DNA Ievel and close to that of Southern blot.The result shows that the method is rapid,simple,sen-sitive and specific.