摘要
报道了Ｉｏｄｏｇｅｎ法碘化标记单克隆抗体（单抗）的最佳条件和对标记单抗免疫活性影响的实验结果，结果表明，当Ｉｏｄｏｇｅｎ：ＭｃＡｂ＝１：４缓冲液ｐＨ＝７．４，旋涡搅拌１０ｍｉｎ时，标记率、放化纯和峰值百分率均较高，标记单抗的免疫活性与标记前比较没有统计学差异（Ｐ＞０．０５）。￣１３１Ｉ－ＨＣＭ８３和￣１２５Ｉ－ＶＣＭＣ与阳性对照结合率为３８．２％和３０．１％，Ｐ／Ｎ为９．１和３０．１。此通用技术具有推广应用价值。
By iodinating monoclonal antibodies(McAbs)with Iodogen,the best labeling conditions and post-label-ing immunoreactivity were investigated.When the weight ratio of Iodogen to McAb was 1:4,pH value ofPBS buffer was 7.4 and vortex agitation was performed for 10 min,the immunoreactivity of post-labelingMcAbs was minimally affected.The binding ratios of ￣125I-HCM83 and￣125I-VCMC with their positive controlwere 38.2%and 30.1%,P/N were equal to 9.1 and 30.1,respectively.These results suggest that thismethod can be in common use for Radioiodination of McAbs.
出处
《科技通报》
1994年第2期120-123,共4页
Bulletin of Science and Technology
基金
浙江省卫生厅资助项目
关键词
单克隆抗体
放射性碘标记
标记单抗
monoclonal antibody
radioiodination
post-labeling immunoreactivity
