摘要
采用国产的微孔玻璃珠吸附层析法分离纯化人干扰素γ／肿瘤坏死因子β重组杂交蛋白，它具有抗病毒和抗肿瘤的双重功能活性，其抗病毒活性为１．７×１０￣６ｕ／ｍｇ蛋白，细胞毒活性为１．４×１０￣６ｕ／ｍｇ蛋白。该杂交蛋白在体外对人宫颈癌细胞株（ＭＥ１８０），人胃癌细胞株（７９０１）和人肺癌细胞株（Ｇ６）均有明显的杀伤作用，并都显著地高于单一的ＩＦＮγ或ＴＮＦβ对这些肿瘤细胞的杀伤。本文还对γＴＮＦβ的抑瘤机理进行了初步探讨。
A recombinant hybrid protein-human interferonγ/tumor necrosis facter β(γTNFβ)was identified andpurified by means of micro-pore glass absorbed chromatography. It possesses dual functional activities of an-ti-virus and anti-tumor. The antivial and cytotoxic activities are 1.7 × 10￣6u/mg protein and 1.4 × 10￣6u/mgprotein respectively.The hybrid protein has appreciable killing effect against human tumor cell lines(such asME 180,7901 as well as G_6)in vitro and its cytotoxic action is significantly stronger than that of single IFNγor TNFβ.The anti-tumor mechanism of this hybrid protein had been investigated.
出处
《科技通报》
1994年第2期69-74,共6页
Bulletin of Science and Technology
基金
国家自然科学基金
关键词
干扰素
重组蛋白
杂交
抗癌药
recombinent hybrid protein(γTNFβ)
micro-pore glass
tumor cell lines
IFNγ
TNFβ
