白细胞介素－1β对原代培养Leydig细胞白细胞介素－αmRNA基因表达的诱导作用

Interleukin-1 β Induces Interleukin-1α Messanger Ribonucleic Acid Expression in Primary Cultures of Leydig Cells

最近发现睾丸中存在ＩＬ－Ｉ样物质，但其来源不明，作者用改良的Ｋｌｉｎｅｆｅｌｔｅｒ方法分离纯种大鼠睾丸Ｌｅｙｄｉｇ细胞，培养于含有人重组ＩＬ－１β的牛血清的ＤＥＭＥ／Ｆ１２培养基中，根据Ｃｈｏｍｃｚｙｎｓ－ｋｉ及Ｓａｃｄｃｈｉ方法，抽提全部细胞的ＲＮＡ，最后用Ｎｏｒｔｈｅｒｎ印迹分析发现ＩＬ－β导致Ｌｅｙｄｉｇ细胞中ＩＬ－１αｍＲＮＡ表达增加，且呈剂量依赖性，脂多糖亦刺激ＩＬ－１αｍＲＮＡ的表达，但佛波脂无此作用．结果表明Ｌｅｙｄｉｇ细胞为ＩＬ－Ｉ的主要来源，它具有自分泌和旁分泌效应．

Recently Interleukin-1(IL-1) like material has been reported to be present in the testis,however,the cel-luiar source and function remains unclear. In this study,we used modified method of Klinefelter to isolate purerat testis leydig cell,cultured with bovine serum in DEME/F12 with human recombinant IL-1β,then extractedtotal cell RNA according to the Chomcynski and Sacchi’s,finally analized by Northern blot.It was found thatIL-1β increases in IL-1α mRNA expression dose dependent in Leydig cell.Lipopolisaccharide(LPS)also stim-ulated IL-1α mRNA experssion,but phorbol ester had no effect.The results indicate that Leydig cells are apotential source of IL-1 which has both autocrine and paracrine effects.