质粒pBR322介导的变形链球菌染色体DNA基因转化研究

Genetic transformation of streptococcus mutans chromosomal DNA by plasmid pBR322

本实验利用质粒ｐＢＲ３２２为克隆载体，成功地进行了变形链球蓖基因片段的转化，探索出了一套简便的分子克隆方法，本方法能可靠地将变形链球菌的ＤＮＡ片段转入大肠杆菌ＴＧ１中，阳性菌株中的重组质粒（携有变形链球菌基因片段）可随细菌的增殖而稳定地传代。通过该实验，我们已获得少量的变链菌基因库。

Abstract It was successful carried out that Strepotococcus mutans 140 (Serotype C) chromosomal DNA was tranformated into E. coli TG1 using plasmid pBR322 as cloning vector. A approch about molecular cloning had been explored by which S.mutans 140 DNA fragment can be efficiently transformated into E. coli TG1. The Transformant contained a recombinant plasmid which carried S.mutans DNA fragment. The recombinant plasmid could exist and replicate stably in E. coli. In this study. a little S. mutans gene bank was constructed.