家蝇卵巢在体外培育中摄取卵黄蛋白

UPTAKE OF YOLK PROTEIN BY HOUSEFLY OVARIES IN VITRO

本文报道家蝇Ｍｕｓｃａｄｏｍｅｓｔｉｃａ卵巢在体外培养条件下，摄取异硫氰萤光素标记的家蝇卵黄蛋白的特点。用Ｇｒａｃ’ｓ培养液标记蛋白浓度为２ｍｇ／ｍｌ，在２７℃条件下培养２小时，卵巢摄取量依赖于培养液中卵黄蛋白浓度和温度．摄取高峰在羽化后４８小时，正值卵母细胞发育阶段进入６—８时期。培养液中加入ＪＨＩＩＩ，能促进摄取，ＪＨＩＩＩ的浓度和摄取量无明显相关性。乌本苷、牛血清蛋白和叠氮钠显著抑制卵巢的摄取活动。

xperiments were performed to study the incorporation of yolk protein labelledwith FITC into housefly follicles in vitro. It was revealed that the rate of yolkprotein incorporation depended on the concentration of yolk protein in the culturemedium and temperature. The optimal condifion for yolk protein incorporation inthis Grace's medium was 2 mg yolk protein per ml culture medium at 27℃ for twohours. The capacity of follicles in yolk protein uptake reached summit when houseflyovaries developed to the stage 6-8 about 48 hours after adult emergence. JH III, ifadded to the culture medium at concentration of 5.9 to 117.3 ng/ul, could increasethe amount of yolk protein incorporated into ovarian follicles. The yolk proteinuptake by follicles in vitro was inhibited by ouabain, BSA or NaN3.