几种昆虫体内保护酶系统活力的研究

A STUDY ON THE ACTIVITIES OF ENDOGENOUS ENZYMESOF PROTECTIVE SYSTEM IN SOME INSECTS

菜粉蝶（Ｐｉｅｒｉｓｒａｐａｃ）体内存在着超氧物歧化酶（ＳＯＤ）、过氧化氢酶（ＣＡＴ）、过氧化物酶（ＰｏＤ）等保护酶系统，ＳＯＤ活力水平与虫龄关系密切。溴氰菊酯处理菜粉蝶后１０分钟，ＳＯＤ、ＣＡＴ及ＰＯＤ的活力水平均高干正常虫体。溴氰菊醋处理褐边绿刺蛾（Ｐａｒａｓａ ｃｏｎｓｏｃｉａ）和揭刺峨（ＴｈｏｓｅａＰａｓｔｏｒｎａｔａ）后，中毒的虫体内ＳＯＤ及ＣＡＴ活力随着中毒程度加重而逐渐上升，接近死亡时又急剧下降；未中毒的活虫体内ＳＯＤ及ＣＡＴ活力水平比对照活虫为高，表明与昆虫耐药性有关。在褐过绿刺蛾和褐刺蛾体内未测得ＰＯＤ。

he endogenous enzymes of protective system including superoxide dismutase(SOD), peroxidase (POD) and catalase (CAT) were found to exist in the larvae ofPieris rapae. The level of SOD activity of the larva was closely correlated with theinstars. After applying deltamethrin to the larvae, the levels of SOD, CAT and PODactivity were found to be higher than that of the control within one hour. Whendeltamethrin was applied to the slug caterpillars Parasa consocia and Thosea pasto-rnata, the SOD and CAT activities were ellhanced gradually with the degree of poiso-ning, but declined rapidly near death. However, the SOD and CAT activities in theslug caterpillars' survived from the deltamethrin treatment were higher than that ofthe control, showing that SOD and CAT activities were correlated with tolerance toinsecticide.