人体肝硬变肝细胞DNA含量的研究

Studies on DNA Content of Human Hepatocytes in Cirrhosis

利用自动化图象分析仪对４８例正常肝、重度慢性活动性肝炎、小结节性肝硬变、大结节及混合结节肝硬变、癌旁肝硬变的肝细胞ＤＮＡ含量进行测定分析，结果：①重度慢性活动性肝炎４Ｎ细胞及双核细胞比率下降；大结节及混合结节肝硬变２Ｎ比率增高；Ｓ期及双核细胞下降，显示细胞分化受抑制，可能与肝癌发生相关，②癌旁肝硬变２Ｎ／４Ｎ比率无明显改变，但≥５Ｎ细胞增高，双核细胞下降，ＤＮＡ直方图增宽，表明肝细胞分化受抑制且有向异倍体发展趋势，具有癌前病变性质，③小结节性肝硬变２Ｎ细胞比率显著下降，Ｓ期及双核细胞比率增高，为分化性代偿修复病变而与肝癌发生无关，肝细胞ＤＮＡ含量的定量分析对于了解肝硬变肝细胞的再生修复状态及患者预后评估将有重要的意义。

he nuclear DNA content of hepatocytes in 48 human cases was measured using anautomated image analyser in normal liver,severe chronic active heptitis(SCAH)micronodular cirrhosis,macronodular cirrhosis and pericarcinoma cirrhosis.The resultsshowed:(1)There was a decrease in tetraploid and binuclear cells in SCAH. Inmacronodular cirrhosis,the percentage of diploid cells was increased, but the S-phase frac-tion valus andhnuclear cells decreased.These changes suggested that the degree of cellulardifferentiation was inhibited.It mav be associated with the carcinogenesis ofhepatocarcinoma.(2) Although the ration of 2N:4N remaind unchanged,an increased frac-tion of≥5N cells and a decreased percentage of binuclear cells was indicated inpericarcinoma cirrhosis.The distribution of DNA contents in these cases was also much widerthan other cirrhosis.It is suggested that the hepatocytes had a tengency to develop theaneuploid cell populations with a reduced degree of differentiation。This cirrhosis pattern maybe a precursor of hepatocarcinoma。3)The micronodular cirrhosis contained a signiflcantlyhigher fraction of S-phase valus and binuclear cells,and a lower diploid eells than other threecirrhosis.It mav be attained increasing degrees of differentiation during liver regeneration after injury,which may be not associated with carcinogenesis So we suggest that the DNA con-tent of liver cirrhosis might objectively reflect the condition of liver proliferation. It may beuseful in the diagnosis and prognosis of cirrhosis.