包虫病人新鲜唾液与冰冻保存唾液中特异性抗体活性的检测

The Detection of the Specific Antibody Activities in Fresh and Frozen Reserved Saliva of Patients with Hydatidosis

采用ELISA对照检测包虫病人新鲜唾液和长期冰冻保存唾液中特异性IgG的活性,证实后者特异性IgG呈明显下降,阳性率由新鲜唾液标本约71％降为24％。为了提高长期冰冻保存包虫病人唾液特异性IgG的阳性检出率,我们采用以10％小牛血清过应孵育长期冰冻保存唾液标本,同时在常规酶复合物稀释液中将NaCl浓度提高至0.5mol/L,以及用4％聚乙二醇(PEG)作为酶复合物稀释液两种方法,可将阳性率由24％提高至57％。

A comparative detection of the specific IgG activities in fresh and frozen reserved saliva of patients with hydatid disease was carried out by ELISA. It is shown that the activities of the specific IgG in saliva of the patients with hydatidosis declines obviously ofter a long period store under -30℃ and the positive percentage falls down to 24% from 71% of positivity in fresh saliva. In order to raise the positivity of the specific IgG in reserved saliva,a few methods were appli-ed,such as:using 10% FCS to incubate the reserved saliva samples overnight,increasing the concentration of NaCl in conventional diluting buffer of enzyme complex to 0. 5 mol/L and using 4% PEG as the diluting buffer of ensyme complex. The positivity can be raised to 57% from original 24% by this method.