用正交试验法选择冬虫夏草菌的最佳发酵培养基

THE SELECTION OF OPTIMUM FERMENTED MEDIUM OF CORDYCEPS SINENSIS BY ORTHOGONAL DESIGN

用正交试验法对冬虫夏草菌（ＣｏｒｄｙｃｅｐｓｓｉｎｅｎｓｉｓＢｅｒｋｓａｃｃ）的发酵培养基进行了研究．考察了葡萄糖、乳精、马铃薯、蚕蛹、氨基酸、复合粉、磷酸二氢钾、硫酸锌、硫酸镁、硫酸亚铁等因素，用Ｌ２７（３１３）表安排正因素、三水平并考虑交互作用的正交试验，对上面各因素的添加量分别做了实验，并对实验结果进行了方差分析，确定了合适的发酵培养基．实验结果表明：冬虫夏草菌的深层发酵最佳培养基应该是：１５％去皮马铃薯、２％葡萄糖、０．８％乳精、１％氨基酸复合粉、０．１％磷酸二氢钾、０．１％硫酸锌、０．１％硫酸镁．

he fermented medium of Cordyceps sinensis studied is by orthogonal design.The factors of glucose.lactose. potato. silkworm chrysalis.amino acid powder.Table L27(313) is used to test four factors with three levels,the interac.non among the factors is examined as well.The results are treated by analysis of variance.The best medium is seleted that 15% potato.2% glucose.0.8% lactose. 1 % amino acid powder.0.1 % KH,PO.0.1% MgSO.0.1 % ZnSO..