耐温酵母TY01－15突变株的诱变育种及其研究

MUTATION AND STUDIES OF THERMOTOLERANT YEAST TY01-15

以热带假丝酵母ＴＹ０１为出发菌株，经多次的物理、化学诱变及传代驯育。采用一种简便而快速的筛选方法，筛选到一株适合于水解液发酵用的耐温突变酵母ＴＹ０１－１５。该突变株在添加营养盐（（ＮＨ＿４）＿２ＳＯ＿４０．３％、ＫＨ＿２ＰＯ＿４０．１５％、ＭｇＳＯ＿４０．１％）、ＰＨ４～５、给予较大的通气量、３７℃条件下正常发酵，其菌体得率达到０．４１ｇ／ｇ，是出发菌株菌体得率（３７℃）的４倍，相当于理论得率的９０％左右，其蛋白质含量为４２％。

A thermotolerant yeast TY01-15 was obtained from Candida tropicalis TY01 as the base start-ing strain by a simple and quick selecion method during the cource of some physical and chemical mutationand domestication. The mutant TY01-l5 ferments well at the temperature of 37℃ and pH 4～5in by-drolyzates replenished with(NH_4)_2 SO_4 0.3%,KH_2 PO_4 0.15%and MgSO_4 0.1%as nutritional saits.Theyield of dry yeast was 0.41 g/g,which was 4 times more than that of starting strain,and equals to 90 per-cent of theoretical yield.Its crude protein content was42%.