摘要
将患有丛枝病的泡桐组培苗进行温度处理,结合茎尖培养用来脱除病原MLO。结果表明,在45℃下,病茎段24—48小时内脱水枯死;在40℃下3周内病苗黄化枯萎;但在30℃和25℃处理的茎段萌发新枝条仍表现典型丛枝症状。而在35℃下处理的茎段,1周后,新生长茎叶外观恢复正常,继续处理至80天,植株仍能正常生长。组织经DAPI染色后荧光显微镜检查显示出在35-40℃下处理的病苗体内MLO4周之内的变化和降解情况。分别剪取35℃下处理29、33、55和80天的组培苗0.5cm的茎尖,转入MS培养基或改良的MS培养基上,在25℃下培养,长成的组培苗一直生长正常。荧光显微镜和电镜检察结果均显示病原MLO已被脱除。
By means of stem tip culture after temperature treatment, the MLO-free plantings were obtained from witches'broom diseased Paulownia tissue culture.1cm long stem segments with two sidebuds were cultured in MS medium,and incubated in the growth chambers at different temperatures.Tee results showed that all the stem segments died in 24-48 hours at 45℃,and within 3 Weeks at 40℃.On the otherhand, the new shoots sprouted from the stem segments, but these still showed typical witches' broom symptoms at 30℃ for 5 weeks. When the stem segments infected with MLO were cultured at 35℃,the apparently healthy shoots sprouted and retained their form more than 178 days.Fluorescence Microscopy revealed that the MLO fluorescence could not be detected in tile sieve tubes of the newly sprouted shoots within 35-40℃ treatments and the intensity of fluorescence in the original stem segments was reduced gradually from 1 to 4 weeks.The stem tips(0.5cm long)were taken as explants from these treated tissue culture at 35℃ for 29,33,55 and 80 days,and then transformed into MS or modified MS medium again.Apparently healthy roots and shoots developed from all these explants at 25℃.The detection with fluorescence microscopy and electron microscopy showed that all such plants had been free from MLO.
出处
《林业科学》
CAS
CSCD
北大核心
1994年第1期34-38,共5页
Scientia Silvae Sinicae
基金
国家自然科学基金
"八五"国家科技攻关项目
关键词
泡桐
丛枝病
温度
茎尖培养
Paulownia witches'broom,Mycoplasma-like organism(MLO),temperature treatment,stem tip culture