喉鳞癌组织淋巴细胞亚群分布的免疫组化研究

An immunohistochemical study of T-lymphocyte subsets in the tissue of laryngeal squamous carcinoma

采用免疫组化ＡＢＣ法检测了２８例喉鳞癌组织中淋巴细胞亚群的分布状态及数量，并与残喉的健侧组织对照研究。发现喉鳞癌局部组织浸润的Ｔ淋巴细胞多于健侧喉正常组织，癌间质的Ｔ细胞多于实质，癌组织中ＣＤ细胞数多于ＣＤ细胞。喉低分化鳞癌和晚期患者癌组织中浸润的ＣＤ和ＣＤ细胞少于高分化癌和早期患者。喉癌局部细胞免疫处于抑制状态，其Ｔ细胞亚群的分布与肿瘤临床分期及组织分化有关，对估计病程发展及预后具有一定价值。

We have studied 28 cases with squamous carinoma of larynx, detecting the lymphocyte subsets in tumour tissue of those cases with immunohistochemistry technique,using monoclonal antibody.The results showed that most of the infiltrating lymphocytes are T-lymphocytes and there are more infiltrating T-lymphocytes in tumour tissue than those in resected normal larynx tissue.The results also showed that there are more T-cells in tumour stroma than those in parenchyma lesion.We found that the main constituents of the T-cells were CD cells and CD cells,especially the latter ones,and the amounts of the former are less than those of the latter.CD, CD in the local tumour tisstie of low atypia and advanced cases are less than those in high atypia and primary patients.It was obvious that the cellular immunity state of local tissue in squamous carcinoma of larynx are disordered and the CD,CD cells are closely related to cellular immunity of laryngeal cancer.