选择性抑制法测定淀粉酶同工酶

Study on determinations of amylase isoenzymes by use of selective inhibitor

本文报道用选择性抑制法结合酶偶联法测定淀粉酶（Ａｍ）同工酶，通过数学推导和实验，建立了结果计算公式和标准曲线。方法线性范围０～１１００Ｕ／Ｌ。批内、批间ＣＶ≤９．９％。参考范围：血清Ｐ－Ａｍ ７～５５Ｕ／Ｌ，８－Ａｍ １．８～６８．３Ｕ／Ｌ；尿Ｐ－Ａｍ ２．５～２２．６Ｕ／ｍｍｏｌＣｒ．Ｓ－Ａｍ ２．２～３１．３Ｕ／ｍｍｏｌＣｒ．本法制备的抑制剂溶液稳定期长，保存、使用方便。

This paper reported the method for determining amylase isoenzymes by using selective inhibitor followed by the enzyme-coupled method.The formulae and the standard curve for calculating activities were established mathematically and experimentally.The linearrange was of 0～1100U/L.The CV.of within-and between-run were of≤9.9%.Reference intervals were as follows:7～ 55U/L and 1.8～68.3U/L for P and S activites in serum,2.5～22.6U/mmol Cr and 2.2～31.3U/mmol Cr for P and S activities in urine respectively.The inhibitor solution prepared had a long term stability and was convenient in storage and use.