氟烷七氟醚对酶诱导缺氧大鼠血浆氨基酸谱的影响（氟烷性肝炎的研究Ⅱ）

The Effects of Halothane,Sevoflurane on Plasma Amino-acid Patterns in Enzyme-induced Hypoxic Rats

雄性ＳＤ大鼠饮用含苯巴比妥（１ｍｇ／ｍｌ）的饮水一周后，随机分为６组，每组６例：ＮＣ，２１％Ｏ２／７９％Ｎ２；ＨＣ，１４％Ｏ２／８６％Ｎ２；ＮＳ，２１％Ｏ２／７９％Ｎ２／１．２ＭＡＣ七氟醚；ＨＳ，１４％Ｏ２／８６％Ｎ２／１．２ＭＡＣ七氟醚；ＮＨ，２１％Ｏ２／７９％Ｎ２／１．２ＭＡＣ氟烷；ＨＨ，１４％Ｏ２／８６％Ｎ２／１．２ＭＡＣ氟烷。吸入时间１ｈ、２４ｈ后用高效液相色谱法测定血浆中１１种游离氨基酸的含量。结果ＮＨ与ＨＨ组酪、精、丝、甘、丙、苏、谷氨酰胺含量升高，而支链氨基酸无明显变化。提示氟烷性肝炎的氨基酸谱的变化类似于急性暴发性肝衰竭。

Male Sprague-Dawley rats were pretreated with phenobarbital and randomly divided into 6 groups arid wereexposed to O2/N2/1.2MAC anesthetics for 1 hr:NC,21%O2/79%N2;HC,14%O2/86%N2;NH,21%O2/79%N2/1.2MAC halothane;HH,14%O2/86%N2/1.2MAC halothane;NS,21%O2/79%N/1.2MAC sevonurane;HS,14%O2/86%N2/1.2MAC sevoflurane. Plasma amino-acid patterns were analysed using HPLC 24 Hrs after exposureconcluded.Plasma concentrations of serine,glutamine, arginine and tyresine were significantly raised in NH,HHgroups. Plasma concentrations of the three branched chain amino-acid valine,isoleucine,and leucine were notmarkedly changed in all groups,These date suggest that the plasma amino-acid patterns of halothane hepatitis isthe same as that of fulminant hepatic failure, The imbalance of the aromatic and the branched chain amino-acidsmay also be a main cause of hepatic coma of halothane hepatitis.