皮脂腺肿瘤凝集素亲合组化研究

Sebaceous gland tumors lectins affinity histochemistry study

本研究采用１７种生物素化的凝集素，应用ＡＢＣ法研究了２３例正常皮肤和２２例皮脂腺良、恶性肿瘤的肿瘤细胞凝集素受体的定位及分布，发现ＬＣＡ在皮脂腺癌的阳性率较高；正常皮脂腺、皮脂腺痣、皮脂腺增生、皮脂腺腺瘤可见胞膜及胞浆的海绵、网状着染；皮脂腺癌中胞浆失去这种规则着染，着染形状及分布极不规则，说明在皮脂腺恶性转化过程中，细胞表面复合糖糖基及细胞的正常结构发生了明显的变化。

The location and distribution of seventeen kinds of lectin-receptors in the sebaceous glands of twenty-three cases of normal controls and twenty-two cases of benign and malignant sebaceous gland tumors were studied by ABC methed. We found that the positive rate of LCA is very high in the patients with sebaceous carcinoma. Cell surface and sponge-like cytoplasmic staining were observed in normal sebaceous glands, sebaceous glands nevus, sebaceous hyperplasia and adenoma of sebaceous glands. In sebaceous carcinoma, diffuse cytoplasmic staining was observed in addition to cell surface staining. It is suggested that the sugar residues on cell surface and normal cell structure have markedly changed in the course of malignant change of sebaceous glands.