高脂血清对主动脉平滑肌细胞增殖的影响

The Proliferative Effect of Hyper lipidemic Sera on the Aortic Smooth Muscle Cells in Vitro

用高脂血清培养猪主动脉平滑肌细胞，观察ＨＬＳ对ＳＭＣ增殖的影响，探讨ＨＬＳ的作用原理。结果表明：ＨＬＳ培养能引起类似动脉粥样硬化的动脉ＳＭＣ增殖。使氚－胸腺嘧啶核苷掺入量增多，形态学上也呈现ＳＭＣ增生，合成功能增强。而且ＳＭＣ的增生，随ＨＬＳ浓度增高而增加。ＳＭＣ增殖与ＨＬＳ激发脂质过氧化过程，使培养液中脂质过氧化物升高，前列环素，环磷酸腺苷含量降低，纤溶酶原激活物抑制物活性增高。

The experimental models of porcine aortic smooth muscle cells (SMC) cultured with hyperlipidemic sera (HLS) were established in order to observe the influence and mechanism of HLS on the proliferation of aortic SMC. The results showed : HLS could faciliate the proliferation of cultured SMC which was close to human atherosclerosis. HLS group had a significantly greater [3H]-thymidine incorporation data than control group with obvious morphological changes of enhanced proliferation and synthesis of SMC. The proliferation of SMC was increased in accordance with the concentration of HLS. HLS evoked the lipid peroxidation, raised MDA content, plasminogen activator inhibitor (PAD activity and dropped 6-keto-PGF1a, cyclic AMP content. These findings suggest that these model of cultured SMC may be usedful in investgation atherosclerosis, and provide some experimental basis.