摘要
本文报告了一种敏感、简单、重复性好、成功率高的非放射性同位素法测定抗细胞性单抗的靶抗原的分子量的技术。此法综合了生物素标记细胞表面抗原、固相免疫分离法、ＳＤＳ－ＰＡＧＥ、免疫印染法和增强的化学发光检测系统等优点，并成功测定了一组抗Ｅｗｉｎｇ氏肉瘤等单克隆抗体相应的细胞表面靶分子的分子量。
A sensitive,simple,reliable non-radioactive technique that combined with biotinylation of cell surfaceantigens,solid phase immunoisolation SDS-PAGE, Western Blotting and enhanced chemiluminescence(ECL)detectionsystem has been developed. The target antigens of a range of monoclonal antibodies against Ewing’s sarcoma have beenidentified using this procedure.
出处
《免疫学杂志》
CAS
CSCD
北大核心
1994年第4期258-262,共5页
Immunological Journal
关键词
分子量
生物素
检测
单克隆抗体
Molecular Weight. Monoclonal Antithey(McAb). Biotinylation, Solid phase immunoisolation,Enhanced chemiluminescence The Heepital for Staff and Workers of Wuhan Steamboiler,Wuhan 430070 University Cinic Muenster,Germany University Clinic Duesseldorf, Germa
