摘要
对10个海岛棉品种的子叶进行了原生质体的游离和培养。培养3~5天的无菌苗子叶经12~16小时的酶解,获得了一定数量的原生质体。不同的酶液浓度组成对原生质体的产量有较大的影响。纯化的原生质体在修改的K3培养基上进行培养,3~5天后再生细胞开始分裂,2个星期后形成小细胞团,l个月后获得了小愈伤组织。
Protoplasts were isolated from cotyledons of 3 to 5 days’old sterile seedings ofGossy pium barbadense. By Enzyming with in 12 to 16 hours,separated and purificated,theprotoplasts were then cultured in modified K3 medium which supplemented with 0.1~0.5mg/L 2,4一D and 0.5~1.0mg/L 2iP, The mixed enzyme solution was composed with l%~2%cellulase(R一10),0.5%~1.O% hemicellulase (Sigina)and 0.5%~1.0%malerozyme(R一10), pH5.8. Protoplasts regencrated cell walls in 2~3days in culture.The first cell divi-sion was apparent after 3~5days and the second cell division was apparent after 5~8days。Cell clusters of l0~100 cells could be seen in two weeks.Microcalli were then obtained afterarnonth.Most microcalli browned and died in late culture.Protoplast yield and viability,celldivision and colonv,Callus formation and propagation were influenced by genotype,age,growth condition of the sterile seeding,enzyme mixture and concentration, and culture medi-um.
出处
《棉花学报》
CSCD
北大核心
1994年第1期34-36,共3页
Cotton Science
关键词
海岛棉
子叶
原生质体
再生愈伤
G. barbadense, cotyledon, protoplast,callus regeneration