氧化苦参碱对LAK细胞活性的影响

Effect of Oxymatrine on LAK Cell Activity

ＬＡＫ细胞具有很强的广谱杀瘤作用，而氧化苦参碱具有较强的免疫抑制作用。本文研究了氧化苦参碱对ＬＡＫ细胞活力的影响，结果表明：氧化苦参碱可抑制ＩＬ－２对小鼠脾细胞的促增殖作用。并且对ＩＬ－２活化ＬＡＫ细胞杀伤Ｐ８１５的能力也有抑制作用。当ＩＬ－２（５００ｕ／ｍｌ）与２００μｇ／ｍｌ的氧化苦参碱共同孵育４ｄ后，可使ＬＡＫ细胞杀瘤能力（在效靶比为１００：１时）的８２．５％被抑制。同时氧化苦参碱本身对Ｐ８１５的增殖无任何效应。

The lymphokine activated killer cell(LAK)has broad spectrum tumor killing ability, and oxymatrine has strong anti-neoplastic activities in vivo.so the effect of oxymatrine on the activities of LAK cells and IL-2 induced proli-feration have been studied,The results showed that oxymatrine can inhibit the IL-2 induced proliferation as well as theP815 killing ability Of LAK cells. The P815 killing ability were 82.5%inhibited if coculture the splenocytes fromC57BL/6 mice with IL-2(500u/ml)and oxymatrine(200ug/ml) for 4 days at effector:target ratio(100:1)。