矽肺大鼠肺泡MΦ上清中生长因子的分离纯化

Identification and Partially Purification of Growth Factor from SilicaExposed Rats’Alveolar Macrophage Supernatants

本文对经ＳｉＯ＿２体内暴露４～５周的矽肺大鼠肺泡巨噬细胞（ＡＭ）上清中促纤维母细胞（ＦＢ）生长的物质进行了分离纯化。由高压液相层析（ＨＰＬｃ）分析结果表明，与正常对照大鼠ＡＭ相比。矽肺大鼠ＡＭ在经体外６ｈ的无血清培养后，上清中含有两个组分的促ＦＢ生长的活性组分。第一组分位于分子量约３５～６０ＫＤａ区间，保留时间为７～９ｍｉｎ，有轻度的促ＦＢ生长活性和ＩＬ－１及ＴＮＦ活性；第二组分位于分子量６～９ＫＤａ区间，保留时间为１２～１４ｍｉｎ，有明显的促ＦＢ生长活性。但不具有任何可检测的ＩＬ－１和ＴＮＦ活性，提示矽肺状态下ＡＭ可能主要通过这种非ＩＬ－１和ＴＮＦ样物质的大量释放而促进ＦＢ的增生，并进而导致肺间质的广泛纤维化。

The growth factor like subetances from the alveotar macrophages(AM) supernatants of silica exposed six-week rats was identified and pertially pruified by TSK Bio-Sil 250 HPLC gel filtration column in present studies. The re-sults indicated that there are extra protein peaks in silica exposed AM supernatant appering in fraction 11～14 comparedwith which of sham control’s, and these fractions can stimulate FB proliferation remarkably. Besides fraction 11 to frac-tion 14.14. the fraction 6～8 can enhance FB growth also. but these fractions have the aetivities of IL-1 and TNF too. Molecular mass ranges of fraction 11～14 and fraction 6～8 estimated by known molecules calibrated are 6～9 KDaand 35～60 KDa respectively. It is concluded that AM from silica exposed rats release other cytokine(s) /growth factor(s)in addition to IL-1 and TNF to medulate inrerstitlal FB’s growth,the biochemistry properties of which remain to beinvestigated.