一种新的短肽点免疫结合试验

Dot Immunobinding Assay of Small Pepidcs on Activatcd Nitrocellulose

采用二乙烯基砜醇、乙二胺和戊二醛活化硝酸纤维素膜，在膜上形成一“结合臂”，该臂含游离的醛基，后者与短肽的游离氨基以肽键结合。以此为基础建立的点免疫结合试验，能区分只有一个残基不同的一组七肽，敏感度达ｐｇ水平。

Nitrocellulose was activated with diviny l sulfone,a specer of ethylenediamine,and glutaraldehyde.Thefree aldehyde group on the spacer were attached the amino group of small poptides, forming peptide bonds。 Identificationof peptides by the dot immunobinding assay based on this was demonstrated with pre-S_(2) poptide analogues:P1. P3 couldbe detected with a sensitivity of 50 pg/cm￣2 ;and P2.P4 wcre differentiated。