Pre－S_（2）蛋白B细胞表位的精细特异性

The Fine Specificity of the Dominant Antibody Binding Site in Pre-S(2)Region of HBsAg

采用我室建立的短肽点免疫结合试验确定Ｐｒｅ－Ｓ＿（２）蛋白Ｂ细胞表位的精细特异性。结果发现Ｐｒｅ－Ｓ＿（２）蛋白的Ｂ细胞识别部位（１４～２４）可进一步区分为两个部分交叠的表位：１４～２１和１８～２４，从而绘出了Ｐｒｅ－Ｓ＿（２）蛋白Ｂ细胞表位图谱。这对设计新一代疫苗及诊断试剂有指导意义。

The new dot immunobinding assay for small peptides established in our laboratory was employed in thestudy of the fine specificity of the dominant antibody binding site in Pre-S_(2)region of HBV surface antign. Two over-lapping epitopes.14～21 and 18～ 24,were identified within the dominant antibody binding site(14～24),and six aminoacid residues,￣(14)D,￣(15)P,￣(20)L,￣(21) Y,￣(18)R,￣(24)A,were proved impertant for those two epitopes, These findings are instructive formolecular design of the new generation HBV vaccines and diagncotic agents.