急性白血病患者血浆肿瘤坏死因子及抑制物

TNF and Inhibitor in Plasma of Patients with Acute Leukemia

利用ＴＮＦ细胞毒生物学活性检测法和ＴＮＦ抑制物（ＴＮＦＩＮＨ）生物活性检测法，检测２２例初治急性白血病患者体内ＴＮＦ和ＴＮＦＩＮＨ水平。急性白血病患者血浆ＴＮＦ水平明显增高。达１１．４２±６．０２ｕ／ｍｌ。抗人ＴＮＦ－α单抗能完全中和Ｍ＿４、Ｍ＿５、Ｍ＿６型急性非淋巴细胞白血病患者血浆ＴＮＦ活性。部分患者血浆中同时存在ＴＮＰ和ＴＮＦＩＮＨ。ＴＮＦ阴性的患者血浆中亦单独存在ＴＮＦＩＮＨ活性，和正常人相比明显增高。其对ｒｈＴＮＦα活性的抑制作用具有剂量依赖关系。测定急性白血病患者体内ＴＮＦ及ＴＮＦＩＮＨ可能为急性白血病的分型和治疗提供新线索。

Using bioassay,we measured levels of tumor necrosis factor necrosis factor(TNF)and tumor necrosis factor inhibitor (TNFINH)in 22 patients with acute leukemia.The plasma levels of TNF cytotoxic activity of the patients were much higher than those Of normal control(11.42±6.020/ml and 1.43±2.29u/ml respectively)The TNF cytotoxic activity found in the plasma of M_4,M_5,M_6types of acute non-lymphocyeic leukemiacould be neutralized by a monoclonal anti-body directed against rhTNFα.There were both TNF and TNFINH activities in the plasma of partial patients and there was only TNFINH acti vity in the plasma of some patien ts。A concentration dependent inhibition of cytotoxicity of rhTNFα by the plasma of these patients was also observed.TNFINH may have an important physiological role in acute leukemia.