摘要
本实验将编码人ＴＮＦａ分子上与其受体相关的部分核苷酸序列缺失，代之以人ＥＧＦ基因ｃＤＮＡ，实现基因重组。转化子经快速细胞破碎法初筛、经Ｄｏｔｂｌｏｔ和Ｓｏｕｔｈｅｒｎｂｌｏｔ鉴定，从中挑选出２０个人ＴＮＦａ－ＥＧＦ阳性重组质粒，为进一步获得重组嵌合人ＴＮＦａ－ＥＧＦ分子和ＴＮＦａ作用机制的深入探讨奠定了初步基础。
In thls study.partial DNA sequence coding the domain of TNFa relating to TNFa receptor was deleted. which is replaced by hu man EG F gene cDNA, So the two genes am fused together. We picked up 20 peresitive humanTNFa-EGF recombinant plasmids with Cracking gel. Dot blot and southern blot,The study lays a primary foundation togetting the recombinant chimeric protein between TNFa and EG F. and to further study of effective mechanism of TNFa.
出处
《免疫学杂志》
CAS
CSCD
北大核心
1994年第3期158-161,共4页
Immunological Journal
关键词
肿瘤坏死因子
表皮生长因子
重组
Tumor necrosis factot a,Epidermal growth factot. Recombinant plasmid
