摘要
本文利用硫酸铵盐析及ＤＥＡＥ纤维素层析提取兔抗人ＣｌｑＩｇＧ．经胃蛋白酶消化及ＳＰＡ－Ｓｅｐｌｉａｒｏｓｅ４Ｂ层析，制备了兔抗人ＣｌｑＩｇＧ（Ｆａｂ＇）＿２．ＳＤＳ－ＰＡＧＥ分析分子量为９３ＫＤ；经Ｃｌｑ－ＥＬＩＳＡ滴定其效价为１０￣（－４）．与人ＩｇＧ无交叉反应；当该制剂预先经人Ｃｌｑ处理后，阻断其与Ｃｌｑ特异结合的能力，在合适的条件下可抑制Ｃｌｑ与Ｒａｊｉ细胞上ＣｌｑＲ的结合。表明用该法制备的兔抗人ＣｌｑＩｇＧ（Ｆａｂ＇）＿２具有较好的纯度及特异性，可用于ＣｌｑＲ功能的研究。
The IgG of rabbit anti-human Clq was extracted by salting out and DEAE-cellulose columm chromatog-raphy,(Fab' )_2 anti-Clq was prepared from extracting IgG by using pepsin digestion and SPA-Sepharose 4B column chro-matography. This material with MW 93 KD was found on SDS-PAGE under un-reducing conditions. The titer of(Fab ' )_2anti-Clq was 10￣(-4) by Clq-ELISA and、was not obeerved cross reaction with human IgG.The results of Clq blocking test and(Fab ')_2 anti-Clq inhibiting test proved that specificity and purity of the(Fab')_2anti-Clq were satisfactory. It could be provided a preparation to study the immune function of ClqR.
出处
《免疫学杂志》
CAS
CSCD
北大核心
1994年第3期188-190,共3页
Immunological Journal
