大鼠DC单抗应用于移植排斥反应的初步研究

Inhibition of Transplanted Rejection by McAbs to Rat Dentritic Cell

应用ＷＺＤ＿（１－５）细胞株分泌的抗大鼠树突状细胞单克隆抗体作微量细胞毒性试验，考察其对大鼠树突状细胞（ｄｅｎｄｒｉｔｉｃｃｅｌｌＤＣ）的特异性杀伤作用；并采用ＷＺＤ＿１单克隆抗体连续给大鼠体内注射，然后作用同种异体皮肤移植后观察移植皮片的存活时间。结果表明ＷＺＤ＿（１－５）单抗对大鼠ＤＣ呈现较高的特异性杀伤活性，杀伤率在６９．３％～７５．３％，ＷＺＤ＿１单抗能有效地延长移植皮片的存活时间，平均存活时间为１６．６ｄ（对照组平均为４．６ｄ．ｐ＜０．００１）。

After establishing five hybridoma cell-lines which can secrete monoclonal antibody to rat dendritic cell.we use these McAbs(WZD_(1～5) to analyze the cytotoxicity for dendriti cells.M. splenocytes and low density cells.Theresults showed that these McAbs have special cytotoxiclty for dendritic cells. Then we injected WZD_1 McAb or NS into SDrat abdomen for 10 days. After this. we transplanted normal wistar rat skin to SD rat as test group and injected WZD_1McAb or NS into SD rat abdomen continuouly for one week.We found that the WZD_1 McAb can extended the time ofskin reJection effioiently(P<0.001)as compared with autologous transplantation controls.